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MSc Informatica Medica
U-Chile
U-Chile / International


Optics, forces & development

| Santiago de Chile 14-29 de Enero 2013, Facultad de Medicina, U-Chile |



Organización

Miguel Concha, LEO-Lab, U-Chile
Steffen Härtel, SCIAN-Lab, U-Chile

Resumen

This practical and theoretical course is aimed at graduate students from Latin America interested in the use of optics and microscopic techniques for in vivo 3D visualisation and analysis of cell and tissue dynamics.

Localización

Facultad de Medicina, Independencia 1027, U-Chile, Santiago

Profesores Participantes

. Roberto Bernal - (U. Santiago, Chi)
. Miguel Concha - (U. Chile, Chi)
. Mauricio Cerda - (U. Chile, Chi)
. Jorg Enderlein - (U. Gottingen, Ger)
. Nikta Fakhri - (U. Gottingen, Ger)
. Steffen Härtel - (U. Chile, Chi)
. Carl-Philipp Heisenberg - (IST, Austria)
. Jorge Jara - (U. Chile, Chi)
. Ulrich Kubitscheck - (U. Bonn, Ger)
. Omar Ramírez - (U. Chile, Chi)
. Florian Rehfeldt - (U. Gottingen, Ger)
. German Reig - (U. Chile, Chi)
. Felipe Santibañez - (U. Chile, Chi)
. Christoph Schmidt - (U. Gottingen, Ger)
. Jan Spille - (U. Bonn, Ger)
. Juan Pablo Staforelli - (CEFOP, Chi)

Students

. Abigail Díaz - (Mexico)
. Paola Lepanto - (Uruguay)
. Cecilia Plavan - (Uruguay)
. Rodolfo Ortiz - (Argentina)
. Talita Pereira - (Brazil)
. Verónica Akle - (Colombia)
. Juan Ulloa - (Chile)
. Álvaro Glavic - (Chile)
. Magdalena Esparza - (Chile)
. Ricardo Figueroa - (Chile)
. Patricio Olguín - (Chile)
. Verónica Bahamondes - (Chile)

-> who is who ...

Tópicos centrales:

. Development of zebrafish and annual fish
. Confocal and spinning microscopy
. Light-sheet microscopy
. Super-resolution microscopy
. Photoactivation and laser ablation
. In vivo electroporation
. Force estimation in cells & tissues
. Optical tweezers
. Particle tracking
. Image processing and analysis

Programa y Poster

Download program  
Download Day by Day program  
Download poster  

Literatura para Seminarios

1. Super-Resolution Microscopy:
 Nanoscopy by Using Blinking Enhanced Quantum Dots
 Superresolution imaging for neuroscience
 Widely accessible method for superresolution fluorescence imaging of living systems

2. Forces and Microscopy:
 Adhesion Functions in Cell Sorting by Mechanically Coupling the Cortices of Adhering Cells
 a-Catenin as a tension transducer that induces adherens junction development
 Deconstructing the Cadherin-Catenin-Actin Complex

3. Differentiation of adult stem cells guided by matrix mechanics:
 Cell responses to the mechanochemical microenvironmentImplications for regenerative medicine and drug delivery
 Hyaluronic acid matrices show matrix stiffness in 2D and 3D dictates cytoskeletal order and myosin-II phosphorylation within stem cells
 Matrix Elasticity Directs Stem Cell Lineage Specification
Supplemental Literature:  Cell locomotion and focal adhesions are regulated by substrate flexibility

4. Image Processing:
  Measurement of Instantaneous Velocity Vectors of Organelle Transport: Mitochondrial Transport and Bioenergetics in Hippocampal Neurons
  Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy (supplementary material  )
  3-D Active Meshes Fast Discrete Deformable Models for Cell Tracking in 3-D Time-Lapse Microscopy.pdf

Maps and Places

Download PDF here  .

Créditos / Credits:
Créditos para el curso se otorgan por la Facultad de Medicina, Universidad de Chile. Las materias podrán ser reconocidas para futuros estudios de postgrado, Diplomado y Magíster en Health Informatics.

Aplicación / Application

Deadline for Application and Fellowship - December 28th 2012
Send CV, letter of intention and references from supervisor/mentor
to Miguel Concha and Steffen Härtel

Manuals & More

  ZEISS Principles of Confocal Microscopy

  Principles of Fluorescence Spectroscopy

  LEICE TCS LSI Brochure

  Huygens Professional User Guide from SVI:

  Intracellular Fluorescent Probe Concentrations by Confocal Microscopy, Finck et al. 1998

  Seeing is believing? Alison J. North, The Journal of Cell Biology, Vol. 172, No. 1, January 2, 2006 918

  The Good, the Bad and the Ugly ! Helen Pearson, NATURE, 447, May 2007

  Measuring and interpreting point spread functions to determine confocal microscope resolution and ensure quality control, Cole et al 2011, NATURE PROTOCOL

  IDL/ScianTimeCalc: 1er Manual de Reconstrución 3D

Clases

Day 1
  Clase 1 G Reig
  Clase 1 S Härtel
  Clase 1: M Concha

Day 2
  Clase 2 S Härtel
  Clase 1 U Kubischeck
  Clase 3 S Härtel

Day 3
  Clase 1 J Jara
  Clase 1 J Spille

Day 4
  Clase 2 U Kubischeck

Day 5 (Friday)
  Clase 2 U Kubischeck

Day 6 (Monday)
  Clase 2 G Reig
  Clase 2 J Jara
  Clase 1 R Bernal
  Clase 2 R Bernal

Day 7 (Tuesday)
  Clase 1 M Cerda

Day 8 (Wednesday)

Day 9 (Thursday)
  Clase 2 M Cerda

Practicos

  Practico 1 M Cerda
  Practico 2 M Cerda




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