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MSc Informatica Medica
U-Chile
U-Chile / International


Optics, Forces and Development



Organizers

Miguel Concha, LEO-Lab, U-Chile
Steffen Härtel, SCIAN-Lab, U-Chile

Summary

We cordially invite you to participate at the international course "Optics, Forces and Development", to be held in Santiago, Chile, on May 5-16th 2014. The aim of the course is to train students, postdocs and young investigators from Latin America in theoretical and practical aspects of in vivo microscopy, and strategies for the visualisation and manipulation of cell and tissue morphodynamics in developing organisms.
The course is organised by the Biomedical Neuroscience Institute (Chile) and QuanTissue (ESF, Europe), and will coincide with the international symposium "Visualisation and manipulation of signals and forces in developing tissues" and the openlecture series on the "Origin of Animal Form in Evolution and Development".

Location

Facultad de Medicina, Independencia 1027, U-Chile, Santiago

Central Topics:

. Principles of optics
. In vivo confocal imaging
. Image processing and analysis
. Force estimation in cells and tissues
. Laser micro-disecction
. Cytoskeletal dynamics
. Model Organisms (fish, fly, frog, mouse)
. Embryo manipulation (fish)
. Stem cells

Teachers, Students, and Program

Teachers, Students, and Program download here:  

Literature

1. Super-Resolution Microscopy:
 Nanoscopy by Using Blinking Enhanced Quantum Dots
 Superresolution imaging for neuroscience
 Widely accessible method for superresolution fluorescence imaging of living systems

2. Forces and Microscopy:
 Adhesion Functions in Cell Sorting by Mechanically Coupling the Cortices of Adhering Cells
 a-Catenin as a tension transducer that induces adherens junction development
 Deconstructing the Cadherin-Catenin-Actin Complex

3. Differentiation of adult stem cells guided by matrix mechanics:
 Cell responses to the mechanochemical microenvironmentImplications for regenerative medicine and drug delivery
 Hyaluronic acid matrices show matrix stiffness in 2D and 3D dictates cytoskeletal order and myosin-II phosphorylation within stem cells
 Matrix Elasticity Directs Stem Cell Lineage Specification
Supplemental Literature:  Cell locomotion and focal adhesions are regulated by substrate flexibility

4. Image Processing:
  Measurement of Instantaneous Velocity Vectors of Organelle Transport: Mitochondrial Transport and Bioenergetics in Hippocampal Neurons
  Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy (supplementary material  )
  3-D Active Meshes Fast Discrete Deformable Models for Cell Tracking in 3-D Time-Lapse Microscopy.pdf

Manuals & More

  ZEISS Principles of Confocal Microscopy
  Principles of Fluorescence Spectroscopy
  LEICE TCS LSI Brochure
  Huygens Professional User Guide from SVI:
  Intracellular Fluorescent Probe Concentrations by Confocal Microscopy, Finck et al. 1998
  Seeing is believing? Alison J. North, The Journal of Cell Biology, Vol. 172, No. 1, January 2, 2006 918
  The Good, the Bad and the Ugly ! Helen Pearson, NATURE, 447, May 2007
  Measuring and interpreting point spread functions to determine confocal microscope resolution and ensure quality control, Cole et al 2011, NATURE PROTOCOL
  IDL/ScianTimeCalc: 1er Manual de Reconstrución 3D

Clases

Day 1
  Clase: Steffen Härtel
Clase Miguel Concha
  Clase Martin Behrndt

Day 2
  Clase 5: Ulrich Kubitscheck A



Day 3




Day 4
  Clase: Jorge Jara A
  Clase: Jorge Jara BC



Day 5

  Clase: Ulrich Kubitscheck B

Day 6
  Clase: Antonio Jacinto

Day 7
  Clase: Roberto Mayor
  Clase: Xavier Trepat

Day 9
  Clase: Phillipp Keller
  Clase: Maria Elena Torres-Padilla




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